Fermentation

What we offer

  • Scale-up and demonstration of fermentation processes 
  • Batch, fed-batch and continuous (with or without cell recycle) fermentations
  • Experience with bacterial, yeast and fungal systems
  • Food grade production (FSSC22000)
  • Optional: methanol (ethanol) dosage on all vessels
  • Process development and optimization
  • First series compound production and custom manufacturing at 15000 L scale
  • Examples of products: industrial enzymes, fine and bulk chemicals, biofuels, biochemicals, bioplastics, biosurfactants, biosolvents, nutraceuticals, food ingredients, bioflavours, proteins… 

Fermentation equipment overview

Batch, fed-batch, continuous.

All fermenters have: six-blade (hollow blade) Rushton impellers, high aeration, baffles and optionally foam breakers, the stainless steel reactors can be pressurized (max 1,4 barg). 

  • 4 x 250 mL glass fermenters with a central control system for parallel operation
  • 4 x 1 L stainless steel (gas) fermenters, max. 10 barg
  • 4 x 2 L glass fermenters with a central control system for parallel operation
  • 4 x 3,6 L glass fermenters with a central control system for parallel operation
  • 4 x 7 L glass fermenters
  • 2 x 10 L glass fermenters
  • 10 x 150 L stainless steel fermenters
  • 2 x 1500 L stainless steel fermenters
  • 2 x 15000 L stainless steel fermenters
  • 1 x 20 L (ATEX)
  • 1 x 85 L (ATEX)
  • 1 x 500 L (ATEX)
  • 1 x 1000 L (ATEX)
  • 1 x 5400 L (ATEX)
  • 4 x 1 L (10 bar pressure)
  • 1 x 10 L (ATEX, 5 bar pressure) 
  • Containerized mobile gas fermentation 10 + 100 L demo unit (under construction)
  • Analytical capabilities: HPLC, GC, GC-MS, fast biochemistry analyzer (YSI), (mass spec) off-gas analysis and data logging
  • 2 x 12000 L feed tanks for the 1500 L and 15000 L fermenters
  • Media preparation room
  • Methanol (or ethanol) feeding to bench scale, 150 L, 1 x 1500 L and 1 x 15000 L fermenters
  • In situ product recovery (ISPR)
  • Nitrogen inertisation and blanketing
  • Equipment for biomass separation: centrifuges, decanter, plate filters, filter presses, membrane filtration equipment (see product recovery and purification folder)
  • A variety of downstream processing equipment to recover and purify the product of interest from the fermentation broth (see product recovery and purification folder)
  • Production of 2G fermentable sugars (see biomass pretreatment folder)
  • Cooling of the fermenters with cooling water from cooling tower and chiller
  • Cell culture lab

Expertise

BBEPP has more than 10 years of experience in optimizing, scaling and transferring your fermentation protocol from the lab to commercial production. We count on an entire team of well-trained and highly motivated fermentation experts both with academic and industrial backgrounds to take your process to the next level!